Journal: OncoTargets and therapy

Article Title: MiR-29c inhibits cell growth, invasion, and migration of pancreatic cancer by targeting ITGB1

doi: 10.2147/OTT.S92758

Figure Lengend Snippet: MiR-29c is significantly downregulated in pancreatic cancer tissues and cell lines. Notes: ( A ) The relative expression levels of miR-29c were evaluated by quantitative real-time polymerase chain reaction in 23 paired tumor tissues and adjacent normal tissues. ( B ) The relative expression levels of miR-29c in four pancreatic cancer cell lines (SW1990, AsPC-1, BxPC-3, PANC-1) and the HPDE cells. * P <0.05. Abbreviation: HPDE, human pancreatic duct epithelial.

Article Snippet: The specific primers for miR-29c (HmiRQP0376) and U6 (HmiRQP9001) were purchased from RiboBio (Guangzhou, PRC).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: OncoTargets and therapy

Article Title: MiR-29c inhibits cell growth, invasion, and migration of pancreatic cancer by targeting ITGB1

doi: 10.2147/OTT.S92758

Figure Lengend Snippet: MiR-29 inhibits proliferation and induces cell-cycle arrest in pancreatic cancer cells. Notes: BxPC-3 and PANC-1 cells were transfected with miR-29c mimics, anti-miR-29c mimics, or corresponding negative control (NC), and the expression levels of miR-29c were analyzed by quantitative real-time polymerase chain reaction ( A ), and then the effects of miR-29c on cell proliferation and the cell cycle were determined using the MTT assay ( B ) and flow cytometry ( C ), respectively. * P <0.05. Abbreviation: OD, optical density.

Article Snippet: The specific primers for miR-29c (HmiRQP0376) and U6 (HmiRQP9001) were purchased from RiboBio (Guangzhou, PRC).

Techniques: Transfection, Negative Control, Expressing, Real-time Polymerase Chain Reaction, MTT Assay, Flow Cytometry

Journal: OncoTargets and therapy

Article Title: MiR-29c inhibits cell growth, invasion, and migration of pancreatic cancer by targeting ITGB1

doi: 10.2147/OTT.S92758

Figure Lengend Snippet: MiR-29 inhibits the invasion and migration of pancreatic cancer cells. Notes: BxPC-3 and PANC-1 cells were transfected with miR-29c mimics, anti-miR-29c mimics, or corresponding negative control (NC), and the effects of miR-29c on cell invasion and migration were determined using transwell assays with Matrigel ( A ) and wound-healing assays ( B ), respectively. * P <0.05. Abbreviation: OD, optical density.

Article Snippet: The specific primers for miR-29c (HmiRQP0376) and U6 (HmiRQP9001) were purchased from RiboBio (Guangzhou, PRC).

Techniques: Migration, Transfection, Negative Control

Journal: OncoTargets and therapy

Article Title: MiR-29c inhibits cell growth, invasion, and migration of pancreatic cancer by targeting ITGB1

doi: 10.2147/OTT.S92758

Figure Lengend Snippet: MiR-29 regulates the expression of MMP2 , MMP9 , and E-cadherin of pancreatic cancer cells. Notes: BxPC-3 and PANC-1 cells were transfected with miR-29c mimics, anti-miR-29c mimics, or corresponding negative control (NC), and the effects of miR-29c on invasion- and migration-related genes ( MMP2 , MMP9 , and E-cadherin) were analyzed by Western blot. * P <0.05.

Article Snippet: The specific primers for miR-29c (HmiRQP0376) and U6 (HmiRQP9001) were purchased from RiboBio (Guangzhou, PRC).

Techniques: Expressing, Transfection, Negative Control, Migration, Western Blot

Journal: OncoTargets and therapy

Article Title: MiR-29c inhibits cell growth, invasion, and migration of pancreatic cancer by targeting ITGB1

doi: 10.2147/OTT.S92758

Figure Lengend Snippet: MiR-29c directly inhibits ITGB1 expression in pancreatic cancer cells. Notes: ( A ) Predicted binding sequences of miR-29c in ITGB1 3′UTR. A fragment of wild type (WT) or mutant (Mut) 3′UTR of ITGB1 was subcloned into the luciferase-reporter vector. Luciferase assays were performed in BxPC-3 cells cotransfected with miR-29c mimics (miR-29c), anti-miR-29c mimics (inhibitor), or corresponding negative control (NC), and a luciferase reporter containing the WT or Mut 3′UTR of ITGB1 . Luciferase activities were measured at 48 hours posttransfection. Similar results were also observed in PANC-1 cells (not shown). ( B ) The expression levels of ITGB1 in BxPC-3 and PANC-1 cells after 48 hours’ transfection were measured by quantitative real-time polymerase chain reaction. * P <0.05. ( C ) The correlation analysis between miR-29c and ITGB1 in 23 PC tumor tissues.

Article Snippet: The specific primers for miR-29c (HmiRQP0376) and U6 (HmiRQP9001) were purchased from RiboBio (Guangzhou, PRC).

Techniques: Expressing, Binding Assay, Mutagenesis, Luciferase, Plasmid Preparation, Negative Control, Transfection, Real-time Polymerase Chain Reaction

Journal: OncoTargets and therapy

Article Title: MiR-29c inhibits cell growth, invasion, and migration of pancreatic cancer by targeting ITGB1

doi: 10.2147/OTT.S92758

Figure Lengend Snippet: ITGB1 overexpression significantly attenuated miR-29c-induced inhibition on cellular migration and invasion of PANC-1 cells. Notes: MiR-29c mimic- or negative control (NC)-treated PANC-1 cells were transfected with ITGB1 expression plasmids lacking 3′UTR to rescue ITGB1 expression or empty vector as control, and the effect of rescued ITGB1 on cell migration or invasion was assessed by wound healing ( A ) or transwell with Matrigel assay ( B ), respectively. * P <0.05 versus the control (miR-29c + vector); # P <0.05 versus miR-29c + vector. Abbreviation: OD, optical density.

Article Snippet: The specific primers for miR-29c (HmiRQP0376) and U6 (HmiRQP9001) were purchased from RiboBio (Guangzhou, PRC).

Techniques: Over Expression, Inhibition, Migration, Negative Control, Transfection, Expressing, Plasmid Preparation, Matrigel Assay

Journal: CNS Neuroscience & Therapeutics

Article Title: Micro RNA ‐29c Correlates with Neuroprotection Induced by FNS by Targeting Both Birc2 and Bak1 in Rat Brain after Stroke

doi: 10.1111/cns.12383

Figure Lengend Snippet: The consequences of in vivo experiment. (A) The expression of miR‐29c in five groups. (B) and (C) The expression of Birc2 mRNA and Bak1 mRNA in rat brains. (D) and (E) The expression of Birc2 protein and Bak1 protein in five groups. MiR‐29c antagomir treatment decreased the infarct volume (F), apoptosis (G) and improved neurological scores (H), while miR‐29c agomir treatment increased the infarct volume (F), apoptosis (G) and aggravated neurological scores (H). *P < 0.05 vs. antagomir control; # P < 0.05 vs. agomor control.

Article Snippet: Intracerebroventricular Infusion of MiR‐29c Antagomir or Agomir MiR‐29c antagomir, agomir, and respective N‐controls (RiboBio) were dissolved in artificial CSF to obtain a concentration of 20 nmol/mL 11 .

Techniques: In Vivo, Expressing